Images from Knox College, Galesburg, IL
Image captured using the LumaScope™ by college students in a molecular biology class. C. elegans expressing GFP in muscle cells with a nuclear localization tag.
"I, and more importantly, the students, found the LumaScope very easy to use. These images were taken by the students with very little practice and not a lot of coaching. The controls were intuitive and easy to use, and we were impressed by the image quality. [Five] lab groups [took] multiple high quality images over the course of about an hour and a half. (Again, on the 1st time using the LumaScope.)" - Dr. Matthew Jones-Rhoades, Assistant Professor of Biology
"The fact that the scope was so easy to move was very helpful. I had it in my lab and Matt got it in the morning and put it in the teaching lab. We only have one microscope that has 40X and above on fluorescence and it's really not appropriate for class students to use. In addition, it is in a research lab, so we usually have to trek the students into another lab to see images." - Dr. Esther Penick, Assistant Professor of Biology
Raw, uncropped images captured using the LumaScope™ by college students in a
molecular biology class. Images 1A & 2A are Live C. elegans undergoing RNAi against a control
gene other than GFP in muscle cells with a nuclear localization
tag. Image 3A is of C. elegans undergoing RNAi against GFP.
Images as seen live on the LumaScope™, saved as gray-scale JPEGs.
1A, 2A & 3A are the same images cropped, with standard automatic optimization of intensity only,
and saved as gray-scale JPEGs. Image 3B becomes much lighter
with optimization of intensity because of the low levels of fluorescence in
these worms.
Downloads
- LumaScope flyer - PDF (0.5MB)
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LumaScope Poster -
PDF (1.3MB)
LumaScope: an Inexpensive, Compact, Sturdy USB-Based Inverted Fluorescence Microscope.
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LumaScope vs. Zeiss -
PDF (1.6MB)
LumaScope vs. Zeiss comparison of bovine pulmonary artery endothelial (BPAE) cells with AlexaFluor 488 phalloidin labeled F-actin (FluoCell Slide #1 F14780; Molecular Probes).
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LumaScope Presentation -
PDF (8.5MB)
Brian Rasnow's Presentation on the LumaScope to the American Association of Physics Teachers July 2010. Included are examples of teaching applications for physics, biophysics, and multidisciplinary science courses
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LumaScope JALA Manuscript -
PDF (1MB)
Download our Author Manuscript published in JALA on the development of the LumaScope.Kahle J, Levin R, Niles W, Rasnow B, Schehlein M, and Shumate C. (2010) An inexpensive simple-to-use inverted fluorescence microscope: a new tool for cellular analysis. JALA (Oct 15(5):355-361) DOI:10.1016/j.jala.2010.06.008