ls720-phase-bkgd-sub-er-160907-318Blue, Green, & Red Fluorescence and Walk-Away Automation

The powerful, new Lumascope 720 (LS720) adds walk-away automation to the many features and high performance of the flagship 3-color LS620. Exquisite XY motion control, motorized focus that allows autofocus and z-stacks, and easy-to-configure software combine to facilitate your microscopy experiments and high content screens. Place the LS720 in your incubator and you have a live cell imaging system at a fraction of the cost of conventional HCS systems. Whether imaging multiple fields in your flasks or 1536 wells of cells with 3 fluorophores in a 48 hour time-lapse, the 720 offers a whole new world of automated microscopy!

 

 

 

ls720-phase-incub-2402br-178Features and Benefits
  • Automated XY stage with autofocus in Z provides images (photos), time-lapse series, and videos recorded directly to your computer
  • Fully functioning microscope empowers users to visualize cells from microplates, flasks, slides or custom labware
  • Modern LED and advanced optical design provide near diffraction-limited (theoretical maximum) resolution
  • Robust software allows set-up and control across many locations, including microplates and custom arrays
  • Versatile and compact design enables use inside cell culture incubators and hoods
  • Detects blue, green and red fluorophores, including BFP, DAPI, FITC, Fluo-4, GFP & mCherry
  • Flip-up deck allows easy objective access
  • Used manually, but also robot compatible (RS485, RS232, 5V digital interfaces)
  • Objective compatibility with standard lenses permits use of your own objectives

 

 

Like the LS620, it utilizes a Semrock Brightline® Pinkel filter set optimized for 405 nm, 488 nm, and 594 nm LED light sources. The 5-filter set is comprised of 3 exciting filters, 1 triple-band emitting filter, and 1 beam-splitting filter. The 720 also contains the same CMOS sensor as the LS620 that is larger than the sensor used in Lumascopes 500 and 400.

 

Excitation and Emission Profiles of Lumascope 720 filters

Semrock 620 chart

 

3-color low mag 280

 

 

 

 

 

 

 

 

Lumascope 720 Specifications 
OpticsBlue, green & red fluorescence; brightfield
Phase ContrastPhase contrast optional
Objective Options2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification
Objective CompatibilitiesRMS-threaded, infinity corrected, 45 mm parfocal distance
Fluorescence FiltersBlue: Excitation 370-410 nm; Emission 429-462 nm
Green: Excitation 473-491 nm; Emission 502-561 nm
Red: Excitation 580-598 nm; Emission 612-680 nm
CameraHigh Sensitivity Monochrome CMOS Sensor; C-mount
Image FormatsJPG, BMP, TIF, GIF, or PNG
Image Size100 x 100 to 1900 x 1900 pixels
Field of View0.9 x 0.9 mm with 20x objective
Video RatesUp to 10 frames/second (fps); with reduced frame size, up to 30 fps
Automated XY StageSBS nest, 6- to 1536-well microplates; microfluidic chambers (contact Etaluma)
SubdeckNo automation; most flasks, dishes, other
Stage Travel Range110 mm x 74 mm
Stage Move Speed25 mm/sec
Well-to-well time (seconds)Image 96 wells: a) 1 color focus & image, 13 min, b) 3 color focus & image, 34 min
Computer RequirementsWindows 7, 8, 8.1, 10; 2 monitors recommended
Automation FriendlySDK available
Power RequirementsUSB for Lumascope; 100-240 V, 50-60 Hz for AutoStage
Dimensions, With Phase37.4 cm W x 43.8 cm D x 46.8 cm H [14.7 in W x 17.3 in D x 18.4 in H]
Weight13 kg (29 lb) without phase; 14 kg (31 lb) with phase
Operating Conditions4°C - 42°C, 5% - 95% RH non-condensing
Downloads and Links

LS720 Product Sheet