4-Channel LS620 Microscope

Blue, Green, and Red Fluorescence, Brightfield, and Optional Phase Contrast

The newest model of the Lumascope 600 Series, the LS620, provides blue, green, and red fluorescence with sharper images and brighter colors than the previous LS600 model. The LS620 also offers easier focus with both coarse and fine adjustment, and the universal C-mount adaptor for use with other cameras.

Lumascopes visualize and capture high resolution images comparable to those from traditional, high-cost microscopes. Lumascopes utilize LED light sources, Semrock filters, advanced optical engineering, and CMOS sensors to provide near diffraction-limited (theoretical maximum) resolution. Powered only by its USB connection, the LS620 easily records your photos, time-lapse series, and videos directly to your computer. And its compact size enables working in challenging locations, including inside incubators, biological safety cabinets, and environmental control workstations.

 

BPAE cells stained to show nuclei (blue), alpha-tubulin (green), and F-actin (red) on an LS 620; LifeTech FluoCells slide #2; 40x Olympus objective.
BPAE cells stained to show nuclei (blue), alpha-tubulin (green), and F-actin (red) on an LS 620; LifeTech FluoCells slide #2; 40x Olympus objective.
Features and Benefits
  • Fully functioning microscope empowers users to visualize cells from slides, microplates, flasks, or dishes
  • Versatile and compact design permits use inside cell culture incubators, tissue culture hoods, and environmental workstations
  • Robust software enables time-lapse studies over seconds, minutes, hours, or days
  • Modern LED and optical design lowers cost and eliminates cumbersome, difficult to maintain laser-driven and arc lamp systems
  • Power and control via USB connection facilitates rapid set up and easy use
  • Custom optimized filter set detects a range of blue, green and red fluorophores, including BFP, DAPI, FITC, Fluo-4, GFP, and mCherry
  • Advanced optics provide excellent resolution in ambient lighting conditions
  • Objective compatibility with standard lenses allows use of your own objectives
  • Optional Phase Contrast Accessory enhances visualization of unstained samples

 

The LS620 is the most powerful digital microscope offered anywhere at this price. Like the LS600, it utilizes a Semrock Brightline® Pinkel filter set optimized for 405 nm, 488 nm, and 594 nm LED light sources. The 5-filter set is comprised of 3 exciting filters, 1 triple-band emitting filter, and 1 beam-splitting filter. The LS620 also contains the same CMOS sensor as the LS600 that is larger than the sensor used in LS520 and LS420.

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 Excitation and Emission Profiles of LS620 filters

Semrock 620 chart

 

 

 

 

 

 

 

 

LS620 Specifications
OpticsBlue, green & red fluorescence; brightfield
Phase ContrastPhase contrast optional
Objective Options2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification
Objective CompatibilitiesRMS-threaded, infinity corrected, 45 mm parfocal distance
Fluorescence FiltersChannel 1: Excitation 370-410 nm; Emission 429-462 nm
Channel 2: Excitation 473-491 nm; Emission 502-561 nm
Channel 3: Excitation 580-598 nm; Emission 612-680 nm
CameraHigh Sensitivity Monochrome CMOS Sensor; C-mount
Image FormatsJPG, BMP, TIF, GIF, or PNG
Image Size100 x 100 to 1900 x 1900 pixels
Field of View0.9 x 0.9 mm with 20x objective
Video RatesUp to 10 frames/second (fps); with reduced frame size, up to 30 fps
Manual XY Stage (optional)Sized for SBS footprint; other labware requires Labware Holders
Labware Holders (optional)Hold slides, 3 sizes Petri dishes, flasks; custom available
Computer RequirementsWindows 7, 8, 8.1, 10
Automation FriendlySDK available
Power RequirementsUSB
Dimensions24 cm W x 14 cm D x 16.5 cm H (9.4 in W x 5.5 in D x 6.5 in H)
Weight3.2 kg (7.1 lb) (without accessories)
Operating Conditions0°C - 42°C, 5% - 95% RH non-condensing
Warranty1 year parts & labor
 
Downloads and Links
Included with the LS620
Compact design allows use inside incubators, hoods, and workstations.

The LS620 comes with the new black anodized deck with tab off the back for mounting the Phase Contrast Accessory (see Products/Phase Contrast & Other Accessories) and accessible deck feature. The accessible deck includes a removable insert that when removed provides easy access to the objective for quick switching to another one.  Also, on the underside of the right end of the deck are 2 tapped holes for mounting the optional Manual XY Stage (see Optional Accessories below).

The LS620 also comes with a USB power/communication cable for connecting to a computer, fluorescence shroud, and external power supply/cord with country-appropriate plug for situations requiring brighter lighting (typically not needed).

The LS620 is controlled by Lumaview 720/600-Series software that can be downloaded at no charge from the Etaluma website (see Resources/Downloads).

Objectives are sold separately (see Products/Objectives).

 

LS620/LS600 Optional Accessories
Phase Contrast Accessory LS620, LS520, LS420 (Olympus pre-centered)Enhances visualization of transparent specimens, including unstained samples
Manual XY Stage for LS620, LS600, LS520, LS420Provides ability to move SBS-dimensioned labware and Labware Holders holding other labware (see below)
Labware Holder for 35 mm Petri dishesFits inside Holder for 60 mm Petri dish or Terasaki plate to achieve SBS footprint
Labware Holder for 60 mm Petri dishes & Terasaki platesSBS outer dimensions
Labware Holder for microscope slides and 50 mm Petri dishesSBS outer dimensions
LumaquantImage analysis software
Image-Pro PremierImage analysis software