Green Fluorescence, Brightfield, and Optional Phase Contrast
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The LS520 Microscope (Lumascope) provides green fluorescence at a very affordable price. LS Microscopes visualize and capture high resolution images comparable to those from traditional, high-cost microscopes. LS Microscopes utilize LED light sources, Semrock filters, advanced optical engineering, and CMOS sensors to provide near diffraction-limited (theoretical maximum) resolution. Powered only by its USB computer connection, the LS520 easily records your photos, time-lapse series, and videos directly to your computer. And its compact size enables working in challenging locations, including inside incubators, biological safety cabinets, and environmental control workstations.

The LS520 is the new upgraded version in the Etaluma LS500-Series. Its deck includes the bracket for the optional Phase Contrast Accessory and also the accessible deck design.

 
Features and Benefits
Alpha-tubulin in BPAE cells by LS500; LifeTech FluoCells slide #2; 40x Olympus objective
Alpha-tubulin in BPAE cells by LS500; LifeTech FluoCells slide #2; 40x Olympus objective
  • Fully functioning microscope empowers users to visualize cells from slides, microplates, flasks, or dishes
  • Versatile and compact design permits use inside cell culture incubators, tissue culture hoods, and environmental workstations
  • Robust software enables time-lapse studies over seconds, minutes, hours, or days
  • Modern LED and optical design lowers cost and eliminates cumbersome, difficult to maintain laser-driven and arc lamp systems
  • Power and control via USB connection facilitates rapid set up and easy use
  • Detects green fluorophores, including FITC, Fluo-4 and GFP
  • Advanced optics provide excellent resolution in ambient lighting conditions
  • Objective compatibility with standard lenses allows use of your own objectives
  • Optional Phase Contrast Accessory enhances visualization of unstained samples

 

The LS520 utilizes a Semrock Brightline®Basic filter set optimized for FITC and other similar fluorophores. The 3-filter set is comprised of 1 excitation filter, 1 emission filter, and 1 beam-splitting filter. The LS520 contains a high sensitivity color CMOS sensor with a 1280 x 800 pixel image. Because the emission filter is limited to 508-552 nanometers, brightfield images are monochromatic.

 

Excitation and Emission Profiles of LS520 filters

Semrock 500 chart

 

 

 

 

 

 

 

 

 

 

LS520 Microscope Specifications
OpticsGreen fluorescence; brightfield
Phase ContrastPhase contrast optional
Objective Options2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification
Objective CompatibilitiesRMS-threaded, infinity corrected, 45 mm parfocal distance
Fluorescence FiltersGreen: Excitation 457-493 nm; Emission 508-552 nm
CameraHigh Sensitivity Monochrome CMOS Sensor
Image FormatsJPG, BMP, TIF, GIF, or PNG
Image Size1280 x 800 pixels
Field of View1.3 x 0.8 mm with 20x objective
Video RatesUp to 8 frames/second (fps)
Manual XY Stage (optional)Sized for SBS footprint; other labware requires Labware Holders
Labware Holders (optional)Hold slides, 3 sizes Petri dishes, flasks; custom available
Computer RequirementsWindows 7, 8, 8.1, 10
Automation FriendlySDK available
Power RequirementsUSB
Dimensions23 cm W x 13 cm D x 13.5 cm H (9.1 in W x 5.1 in D x 5.3 in H)
Weight2.5 kg (5.4 lb) (without accessories)
Operating Conditions0°C - 42°C, 5% - 95% RH non-condensing
Warranty1 year parts & labor

 

Downloads & Links
Included with the LS520

The LS520 comes with the new standardized deck that includes both the tab for mounting the Phase Contrast Accessory and the removable insert for easy objective change. On the underside of the left end of the deck are 2 tapped holes for mounting the optional Manual XY Stage (see Optional Accessories below).

The LS520 also comes with a USB power/communication cable for connecting to a computer, fluorescence shroud, and external power cord with country-appropriate plug for situations requiring brighter lighting (not typically needed).

The LS520 is controlled by Lumaview 500/400 software that can be downloaded at no charge from the Etaluma website (see Resources/Downloads).

Objectives are sold separately (see Products/Objectives).

 

LS520 Microscope Optional Accessories
Phase Contrast Accessory LS620/LS520/LS420 (Olympus pre-centered)Enhances visualization of transparent specimens, including unstained samples
Manual XY Stage for LS620, LS600, LS520, LS420Provides ability to move SBS-dimensioned labware and Labware Holders holding other labware (see below)
Labware Holder for 35 mm Petri dishesFits inside Holder for 60 mm Petri dishes & Terasaki plates to achieve SBS footprint
Labware Holder for 60 mm Petri dishes & Terasaki platesSBS outer dimensions
Labware Holder for microscope slides & 50 mm Petri dishesSBS outer dimensions
Labware Holder for 4 microscope slides in parallelSBS outer dimensions
LumaquantImage analysis software
Image-Pro PremierImage analysis software