A Dramatic New Concept in High Resolution, Inverted Fluorescence Microscopy

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BPAE cells stained to show nuclei (blue), alpha-tubulin (green), and F-actin (red) on an LS 620; LifeTech FluoCells slide #2; 40x Olympus objective.
BPAE cells stained to show nuclei (blue), alpha-tubulin (green), and F-actin (red) on an LS 620; LifeTech FluoCells slide #2; 40x Olympus objective

LS Microscopes (Lumascopes) are highly versatile, compact inverted fluorescence microscopes that provides high resolution images comparable to those from traditional, high-cost microscopes. The LS Microscope design was conceived and developed by working scientists who realized that a large proportion of everyday fluorescence microscopy involves imaging and measuring the presence, health, and signal of labeled cells. Traditional inverted microscopes are expensive, complicated, and loaded with features that are not required for these tasks. LS Microscopes focus on: quality images, versatility, compact design, ease of use, and amazingly low price.

LS Microscopes utilize LED light sources, Semrock filters, advanced optical engineering, and CMOS sensors to provide near diffraction-limited (theoretical maximum) resolution. Powered only by a USB connection, LS Microscopes easily record your photos, time-lapse series, and live videos directly to your computer. And their compact size enable working in challenging locations, including inside incubators, biological safety cabinets, and environmental control workstations with remote control and image monitoring.

The inverted design accommodates microplates, flasks, dishes and custom labware in addition to slides that you can adjust by the Manual XY Stage (on the LS620, LS520 or LS420; sold separately) or control manually or automatically on the automated LS720. LS Microscopes come with Lumaview software that sends images directly to your computer via a USB cable, eliminating the need for on-board image storage and processing and contributing to the compact size. Lumaview enables time-lapse studies over seconds, minutes, hours, or days. Live videos can be recorded at up to 30 frames per second.

Comparison of LS Microscope Models
FeaturesLS520LS620LS720
OpticsGreen fluorescence; brightfieldBlue, green & red fluorescence; brightfieldBlue, green & red fluorescence; brightfield
Phase ContrastPhase contrast optional (Olympus)Phase contrast optional (Olympus)Phase contrast optional (Olympus)
Objective Options1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification
Objective CompatibilitiesRMS-threaded, infinity corrected, 45 mm parfocal distanceRMS-threaded, infinity corrected, 45 mm parfocal distanceRMS-threaded, infinity corrected, 45 mm parfocal distance
Fluorescence FiltersGreen: Ex 457-493, Em 508-552 nmBlue: Ex 370-410, Em 429-462 nm; Green: Ex 473-491, Em 502-561 nm; Red: Ex 580-598, Em 612-680 nmBlue: Ex 370-410, Em 429-462 nm; Green: Ex 473-491, Em 502-561 nm; Red: Ex 580-598, Em 612-680 nm
CameraHigh Sensitivity CMOS SensorHigh Sensitivity Monochrome CMOS Sensor, 5 megapixel, C-mountHigh Sensitivity Monochrome CMOS Sensor, 5 megapixel, C-mount
Image FormatsJPG, BMP, TIF, or PNGJPG, BMP, TIF, or PNGJPG, BMP, TIF, or PNG
Image Size1280 x 800 pixels100x100 to 1900x1900 pixels100x100 to 1900x1900 pixels
Field of View1.3 x 0.8 mm with 20x objectiveUp to 0.78 x 0.78 mm at 20xUp to 0.78 x 0.78 mm at 20x
Video RatesUp to 8 fpsUp to 10 fps; with reduced frame size, up to 30 fpsUp to 10 fps; with reduced frame size, up to 30 fps
StageManual XYManual XYAutomated XYZ, SBS nest, 6- to 1536-well microplates; microfluidic chambers (contact Etaluma)
SubdeckNANANo automation; most flasks, dishes, other
Well-to-well time (seconds)NANAImage 96 wells: a) 1 color focus & image: 13 min; b) 3 color focus & image: 34 min
Computer RequirementsWindows 7, 8, 8.1, 10; Core i5, SSDWindows 7, 8, 8.1, 10; Core i5, SSDWindows 7, 8, 8.1, 10; Core i5, SSD
Automation FriendlySDK availableSDK availableSDK available
Power RequirementsUSBUSBUSB for LS; 100-240 V, 50-60 Hz for autostage
Dimensions23 W x 13 D x 13.5 H cm (9.1 W x 5.1 D x 5.3 H inches)24 W x 14 D x 16.5 H cm (9.4 W x 5.5 D x 6.5 H inches)37.4 W x 43.8 D x 23.9 H cm (14.7 W x 17.3 D x 9.4 H inches)
Weight2.3 kg (5 lb)3.2 kg (7.1 lb)11 kg (25 lb)
Operating Conditions0°C - 42°C, 5% - 95% RH non-condensing0°C - 42°C, 5% - 95% RH non-condensing0°C - 42°C, 5% - 95% RH non-condensing

 

LS Microscopes Can Be Used in Many Applications
  • Compact design allows use inside incubators, hoods, and workstations.
    Compact design allows use inside incubators, hoods, and workstations.

    Live cell imaging

  • Cell growth and confluence
  • Cell migration and wound healing
  • Determining transfection efficiency
  • Tracking and quantifying protein expression
  • Following cell differentiation
  • High Content Analysis
  • Intravital studies
  • Photomicroscopy in locations without AC power

See Applications for additional examples and results.

 

 

Original Equipment Manufacturer (OEM) Opportunities

LS Microscopes or their components can be incorporated into other instrumentation. Etaluma provides optical components, microscopy systems, private-label LS Microscopes and accessories through OEM agreements. Custom designs are also possible by Etaluma’s experienced Engineering team.  See OEM Opportunities for examples of possible OEM products.