A Dramatic New Concept in High Resolution, Inverted Fluorescence Microscopy
LS Microscopes (Lumascopes) are highly versatile, compact inverted fluorescence microscopes that provides high resolution images comparable to those from traditional, high-cost microscopes. The LS Microscope design was conceived and developed by working scientists who realized that a large proportion of everyday fluorescence microscopy involves imaging and measuring the presence, health, and signal of labeled cells. Traditional inverted microscopes are expensive, complicated, and loaded with features that are not required for these tasks. LS Microscopes focus on: quality images, versatility, compact design, ease of use, and amazingly low price.
LS Microscopes utilize LED light sources, Semrock filters, advanced optical engineering, and CMOS sensors to provide near diffraction-limited (theoretical maximum) resolution. Powered only by a USB connection, LS Microscopes easily record your photos, time-lapse series, and live videos directly to your computer. And their compact size enable working in challenging locations, including inside incubators, biological safety cabinets, and environmental control workstations with remote control and image monitoring.
The inverted design accommodates microplates, flasks, dishes and custom labware in addition to slides that you can adjust by the Manual XY Stage (on the LS620, LS520 or LS420; sold separately) or control manually or automatically on the automated LS720. LS Microscopes come with Lumaview software that sends images directly to your computer via a USB cable, eliminating the need for on-board image storage and processing and contributing to the compact size. Lumaview enables time-lapse studies over seconds, minutes, hours, or days. Live videos can be recorded at up to 30 frames per second.
|Comparison of LS Microscope Models|
|Optics||Green fluorescence; brightfield||Blue, green & red fluorescence; brightfield||Blue, green & red fluorescence; brightfield|
|Phase Contrast||Phase contrast optional (Olympus)||Phase contrast optional (Olympus)||Phase contrast optional (Olympus)|
|Objective Options||1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification||1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification||1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification|
|Objective Compatibilities||RMS-threaded, infinity corrected, 45 mm parfocal distance||RMS-threaded, infinity corrected, 45 mm parfocal distance||RMS-threaded, infinity corrected, 45 mm parfocal distance|
|Fluorescence Filters||Green: Ex 457-493, Em 508-552 nm||Blue: Ex 370-410, Em 429-462 nm; Green: Ex 473-491, Em 502-561 nm; Red: Ex 580-598, Em 612-680 nm||Blue: Ex 370-410, Em 429-462 nm; Green: Ex 473-491, Em 502-561 nm; Red: Ex 580-598, Em 612-680 nm|
|Camera||High Sensitivity CMOS Sensor||High Sensitivity Monochrome CMOS Sensor, 5 megapixel, C-mount||High Sensitivity Monochrome CMOS Sensor, 5 megapixel, C-mount|
|Image Formats||JPG, BMP, TIF, or PNG||JPG, BMP, TIF, or PNG||JPG, BMP, TIF, or PNG|
|Image Size||1280 x 800 pixels||100x100 to 1900x1900 pixels||100x100 to 1900x1900 pixels|
|Field of View||1.3 x 0.8 mm with 20x objective||Up to 0.78 x 0.78 mm at 20x||Up to 0.78 x 0.78 mm at 20x|
|Video Rates||Up to 8 fps||Up to 10 fps; with reduced frame size, up to 30 fps||Up to 10 fps; with reduced frame size, up to 30 fps|
|Stage||Manual XY||Manual XY||Automated XYZ, SBS nest, 6- to 1536-well microplates; microfluidic chambers (contact Etaluma)|
|Subdeck||NA||NA||No automation; most flasks, dishes, other|
|Well-to-well time (seconds)||NA||NA||Image 96 wells: a) 1 color focus & image: 13 min; b) 3 color focus & image: 34 min|
|Computer Requirements||Windows 7, 8, 8.1, 10; Core i5, SSD||Windows 7, 8, 8.1, 10; Core i5, SSD||Windows 7, 8, 8.1, 10; Core i5, SSD|
|Automation Friendly||SDK available||SDK available||SDK available|
|Power Requirements||USB||USB||USB for LS; 100-240 V, 50-60 Hz for autostage|
|Dimensions||23 W x 13 D x 13.5 H cm (9.1 W x 5.1 D x 5.3 H inches)||24 W x 14 D x 16.5 H cm (9.4 W x 5.5 D x 6.5 H inches)||37.4 W x 43.8 D x 23.9 H cm (14.7 W x 17.3 D x 9.4 H inches)|
|Weight||2.3 kg (5 lb)||3.2 kg (7.1 lb)||11 kg (25 lb)|
|Operating Conditions||0°C - 42°C, 5% - 95% RH non-condensing||0°C - 42°C, 5% - 95% RH non-condensing||0°C - 42°C, 5% - 95% RH non-condensing|
LS Microscopes Can Be Used in Many Applications
Live cell imaging
- Cell growth and confluence
- Cell migration and wound healing
- Determining transfection efficiency
- Tracking and quantifying protein expression
- Following cell differentiation
- High Content Analysis
- Intravital studies
- Photomicroscopy in locations without AC power
See Applications for additional examples and results.
Original Equipment Manufacturer (OEM) Opportunities
LS Microscopes or their components can be incorporated into other instrumentation. Etaluma provides optical components, microscopy systems, private-label LS Microscopes and accessories through OEM agreements. Custom designs are also possible by Etaluma’s experienced Engineering team. See OEM Opportunities for examples of possible OEM products.